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Preliminary Results of Combining Low Frequency Low Intensity Ultrasound and Liposomal Drug Delivery to Treat Tumors in Rats
Pitt, William G. Husseini, Ghaleb Roeder, Beverly L. Dickinson, David J. Warden, David R. Hartley, Jonathan M. Jones, Peter W.
Pitt, William G.
Husseini, Ghaleb
Roeder, Beverly L.
Dickinson, David J.
Warden, David R.
Hartley, Jonathan M.
Jones, Peter W.
Date
2011
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Type
Peer-Reviewed
Article
Published version
Article
Published version
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49RatLip(JNN).PDF
Adobe PDF, 802.44 KB
Description
Abstract
Ultrasound is a convenient trigger for site-specific drug delivery in cancer therapy. Nanosized liposomes formulated from soy phosphatidyl choline, cholesterol, 1,2-distearoyl-sn-glycero- 3-phosphoethanolamine-N-[carboxy(polyethylene glycol)-2000] and alpha-tocopherol were loaded with Doxorubicin (Dox) using a pH gradient. The liposomal suspension was infused through the tail vein of BDIX rats possessing bilateral intradermal DHD/K12 tumors on their hind legs. Then 20-kHz ultrasound was applied to only one of the tumors for 15 minutes. This therapy was repeated weekly for 4 weeks. The results showed that in five of six rats, the tumors regressed to non-measurable size within 4 weeks. A paired comparison of the normalized size of the insonated and non-insonated tumors in the same rat indicated that the insonated tumors were smaller (p < 0 0001, n = 6 rats, 21 pairs). This observation has significant potential for non-invasive site-specific therapy of solid tumors.