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Drug delivery in pluronic micelles: effect of high-frequency ultrasound on drug release from micelles and intracellular uptake
Marin, Alexander Sun, Hao Husseini, Ghaleb Pitt, William G. Christensen, Douglas A. Rapoport, Natalya Y.
Marin, Alexander
Sun, Hao
Husseini, Ghaleb
Pitt, William G.
Christensen, Douglas A.
Rapoport, Natalya Y.
Date
2002
Advisor
Type
Peer-Reviewed
Article
Postprint
Article
Postprint
Degree
Citations
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8HighFrequency(JCR).pdf
Adobe PDF, 732.43 KB
Description
Abstract
The effect of high-frequency ultrasound on doxorubicin (DOX) release from Pluronic micelles and intracellular DOX uptake was studied for promyelocytic leukemia HL-60 cells, ovarian carcinoma drug-sensitive and multidrug-resistant (MDR) cells (A2780 and A2780/ADR, respectively), and breast cancer MCF-7 cells. Cavitation events initiated by high-frequency ultrasound were recorded by radical trapping. The onset of transient cavitation and DOX release from micelles were observed at much higher power densities than at low-frequency ultrasound (20–100 kHz). Even a short (15–30 s) exposure to high-frequency ultrasound significantly enhanced the intracellular DOX uptake from PBS, RPMI 1640, and Pluronic micelles. The mechanisms of the observed effects are discussed.