Pitt, William G.Husseini, GhalebRoeder, Beverly L.Dickinson, David J.Warden, David R.Hartley, Jonathan M.Jones, Peter W.2020-08-272020-08-272011Pitt, W. G., Husseini, G. A., Roeder, B. L., Dickinson, D. J., Warden, D. R., Hartley, J. M., & Jones, P. W. (2011). Preliminary results of combining low frequency low intensity ultrasound and liposomal drug delivery to treat tumors in rats. Journal of Nanoscience and Nanotechnology, 11(3), 1866–1870. https://doi.org/10.1166/jnn.2011.31171533-4880http://hdl.handle.net/11073/19736Ultrasound is a convenient trigger for site-specific drug delivery in cancer therapy. Nanosized liposomes formulated from soy phosphatidyl choline, cholesterol, 1,2-distearoyl-sn-glycero- 3-phosphoethanolamine-N-[carboxy(polyethylene glycol)-2000] and alpha-tocopherol were loaded with Doxorubicin (Dox) using a pH gradient. The liposomal suspension was infused through the tail vein of BDIX rats possessing bilateral intradermal DHD/K12 tumors on their hind legs. Then 20-kHz ultrasound was applied to only one of the tumors for 15 minutes. This therapy was repeated weekly for 4 weeks. The results showed that in five of six rats, the tumors regressed to non-measurable size within 4 weeks. A paired comparison of the normalized size of the insonated and non-insonated tumors in the same rat indicated that the insonated tumors were smaller (p < 0 0001, n = 6 rats, 21 pairs). This observation has significant potential for non-invasive site-specific therapy of solid tumors.en-USUltrasoundDrug DeliveryLiposomesDoxorubicinBDIX RatsRumor ModelPeer-Reviewed10.1166/jnn.2011.3117